FIGURE 4.

Affinity determination of individual sites from CSPs. NMR CSPs of ¹⁵  N‐β‐LgA (200 μM) titrated with AOS (1.6, 3.75, and 10 mM for DP 6, 5, and 4, respectively) as a function of ligand concentration. CPS induced by M6 are fitted to a hyperbolic binding curve to obtain K_D for residues with CSP > AVG + 1SD. (a) Sites 1 + 3, (b) site 2, (c) site 4. (d) K_D values of individual residues with CSP > AVG + 1SD extracted from NMR (Figure S3). Gray dotted lines correspond to global K_D values determined by ITC