Fig. 1.

Normalized melting curves of positive control RNAs for the N501 site. Normalized melting curve plots for the N501 site without the addition of the wild-type fragment (A) and with the addition of the wild-type fragment (B) were acquired using standard fragments of 10⁶ copies/mL wild-type (dashed black line), 10⁸ copies/mL N501Y mutant (solid brown line), 10⁶ copies/mL N501Y mutant (dashed red line), and 10³ or 10⁴ copies/mL N501Y mutant (solid purple line).