Table 1.

Results of the high-resolution melting analyses at the N501 site using clinical samples.

Without the addition of the wild-type fragment (conventional HRM-based assay)
HRM result	Sanger sequence
	N501 (n = 105)	N501Y (n = 104)
N501	105	53
N501Y	0	39
Unidentified	0	12

With the addition of the wild-type fragment (modified HRM-based assay)
HRM result	Sanger sequence
	N501 (n = 105)	N501Y (n = 104)
N501	105	10
N501Y	0	78
Unidentified	0	16

Results of the combined assays
	N501 (n = 105)	N501Y (n = 104)
Both assays positive	105	21
Either assay positive	0	76
Both assays negative	0	7