Figure 4.

Summary of amiloride-sensitive currents (ΔI_ami) values from similar experiments as shown in Fig. 3. Connecting tubule (CNT)/cortical collecting duct (CCD) and late distal convoluted tubule (DCT2)/CNT data are summarized in A and B, respectively. Individual measurements, medians, and quartiles are shown. In both diagrams, the left and middle columns show all ΔI_ami values obtained from control mice and mineralocorticoid receptor (MR) knockout (KO) mice (MR KO overall), respectively. Data points from female and male animals are depicted with circles and triangles, respectively. No obvious sex effect was noticed in any experimental group. Individual ΔI_ami values from control mice are depicted with black symbols. Red symbols are used for ΔI_ami values from MR KO mice in which ΔI_ami in the CNT/CCD did not exceed 10 pA in any measurement. Blue symbols indicate ΔI_ami values obtained from mice not meeting this criterion. For clarity, the red ΔI_ami values are shown separately in the right columns called “MR KO max. < 10 pA” in A and “MR KO max. < 10 pA in CNT/CCD” in B. Numbers of mice (N) and individual measurements (n) are given below each group of mice. The age of control mice (5 females and 6 males) ranged from 36 to 68 days, and the age of MR KO mice (12 females and 11 males) ranged from 35 to 65 days.