Figure 5.
Single-channel open probability (Po) of the epithelial Na+ channel (ENaC) in the late distal convoluted tubule (DCT2)/connecting tubule (CNT) from mineralocorticoid receptor (MR) knockout (KO) mice is similar to that in control mice. A and B: representative outside-out patch-clamp recordings from the DCT2/CNT of a male control mouse (A) and a female MR KO mouse (B). Black bars indicate amiloride (Ami) application; dotted lines delineate intervals used for the analysis of the distribution of current levels, the construction of amplitude histograms, and the calculation of channel activity (NPo). C and D: observed (open bars) and best-fit (black bars) probability distribution for the current levels of the traces shown in A and B, respectively; corresponding estimated Po values and estimated numbers of channels (N) are given. E: summary of estimated Po values in control (N = 5 animals, n = 7 individual recordings) and MR KO mice (N = 9 animals, n = 11 individual recordings). F: summary of estimated number of channel values from the same experiments. Individual observations with medians and quartiles are shown. The data points from the representative traces shown in A and B are encircled.