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. 2023 Jan 18;9:12. doi: 10.1038/s41420-023-01320-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Electrophoresis bands of SPHK2, NLRP3, Caspase-1, and ASC from lysates of PBMCs in different groups. GAPDH acted as an internal reference. B Protein levels quantified by densitometry using Image J software were shown as bar graphs. C mtROS levels were measured in supernatant lysates of PBMCs from subjects. Data were expressed as mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001; N.S., not significant.