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. 2022 Dec 10;10:rbac100. doi: 10.1093/rb/rbac100

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — N-cadherin/β-catenin expression and distribution are regulated by paxillin. (A) STRING analysis of the relationship between paxillin and N-cadherin (or E-cadherin)/β-catenin. N-cadherin (gene name was cdh2), E-cadherin (gene name was cdh1) and β-catenin (gene name was ctnnb1). (B) Representative immunofluorescence images of N-cadherin distribution in DPCs after treatment with siPXN. (C) N-cadherin expression levels detected by western blotting after the treatment of siPXN and the relative quantification (n = 3). (D) Representative immunofluorescence images of β-catenin distribution after treatment with siPXN (n = 30). (E) The mean fluorescence intensity of β-catenin in nuclei with or without treatment with siPXN. (F) The linear profile of β-catenin intensity distribution based on the immunostaining image. Data are means ± SD, **P < 0.01, ***P < 0.001.