FIG. 3.

Fibronectin-binding activities of isolates representing different B. burgdorferi genospecies. Proteins from the spirochete lysates were separated by SDS-PAGE on a 12% polyacrylamide gel. The proteins were detected by staining with Coomassie blue (A) or transferred to a nitrocellulose membrane for ligand blotting with alkaline phosphatase-labeled human fibronectin (B). Isolates B31, IP90, ACA1, and DN127 have been genotyped as B. burgdorferi sensu stricto, B. garinii, B. afzelii, and B. bissettii, respectively. The weak fibronectin-binding activity of strain ACA1 was evident in the original ligand blot but may not be clearly visible in photograph (B). Sizes of the molecular weight markers (MWS) are shown in kilodaltons.