FIG. 3.

Phase-contrast microscopy of Wish cells incubated for 3 h at 37°C in 5% CO₂ atmosphere with RPMI medium in the absence (A) or in the presence (B) of 20 μM suramin; with heat-treated filtered cRPMI, conditioned for 2 h by 6 × 10⁶ A. castellanii trophozoites, in the absence (C) or in the presence (D) of 20 μM suramin; or with RPMI containing 20 μM ADP₀ in the absence (E) or in the presence (F) of 20 μM suramin. Magnification, × 400.