Table 3.
SH-SY5Y cell viability (%) after treatment with H2O2 (100 µM) or mixture of rotenone (30 µM) and oligomycin A (10 µM) at selected concentrations of 3e.
| Concentrations |
||||||
|---|---|---|---|---|---|---|
| Cells without compounds | Compound | 10 µM | 1 µM | 0.1 µM | 0.01 µM | |
| H2O2 100 µM |
% cell viability | |||||
| 84.27 ± 0.03 | 3e | 41.11 ± 3.60 | 95.44 ± 3.09 | 96.75 ± 4.23 | 90.57 ± 7.65 | |
| Trolox | 99.22 ± 3.30 | 99.38 ± 9.81 | 98.62 ± 1.36 | 96.41 ± 1.51 | ||
| % neuroprotection | ||||||
| 3e | 71 | 79.34 | 40.04 | |||
| Trolox | 95.05 | 96.03 | 91.22 | 77.16 | ||
| 0.1 µM | 0.01 µM | 0.001 µM | 0.0001 µM | |||
| Rotenone/Oligomycin A 30/10 µM | % cell viability | |||||
| 46.77 ± 2.17 | Pre-incubation 3e | 48.05 ± 1.73 | 50.84 ± 7.02 | 53.40 ± 7.03 | 56.15 ± 6.55 | |
| Pre-incubation Trolox | 44.26 ± 3.73 | 46.38 ± 2.06 | 47.91 ± 4.48 | 46.23 ± 3.65 | ||
| 47.91 ± 4.02 | Co-incubation 3e | 46.76 ± 6.52 | 49.38 ± 4.59 | 52.43 ± 6.60 | 54.55 ± 6.72 | |
| Co-incubation Trolox | 48.09 ± 8.01 | 50.34 ± 8.11 | 50.83 ± 6.98 | 42.11 ± 6.28 | ||
| % neuroprotection | ||||||
| Pre-incubation 3e | 2.41 | 7.65 | 12.46 | 17.64 | ||
| Pre-incubation Trolox | – | – | 2.15 | – | ||
| Co-incubation 3e | – | 2.82 | 8.67 | 12.75 | ||
| Co-incubation Trolox | 0.35 | 4.67 | 5.60 | – | ||
Cell viability was determined by the MTT test. The results were expressed as mean ± SD. The experiment was performed in quadruplicate and repeated three times.