Figure 6.

Effect of Epicoccum nigrum isolates on wheat PR gene expression. Tops of the 2-day-old wheat coleoptiles were cut off and the conidia suspensions of E. nigrum A33 (A) and E. nigrum A39 (B) were injected into them. Coleoptiles were then cultured at 25 °C in the growth chamber for 24 h. The tops of the coleoptiles were removed again and the conidia suspension of Fusarium graminearum was inoculated. Coleoptile were then cultured at 25 °C for another 24 h. The red arrow represents the injection time point of the isolate and the black arrow represents the inoculation time point of F. graminearum. Gene expression levels were normalized to β-actin gene expression.