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The primers sequences used in this study.
| Antibiotic | Gene | Nucleotide sequence (5’-3’) | Annealing temperature | Product length (bp) | Reference |
|---|---|---|---|---|---|
| Clarithromycin | rrl | F: CGGGAWYCGGYCGCAGAAC R: CCAGGTCTGGCCTATCRAWC |
60 | 1110 | Designed in this study |
| Rifampin | rpoB | F: GGGAACGGATGACCACTCA R: GCGGTACGGCGTCTCGATGAAG |
53 | 350 | Designed in this study |
| Moxifloxacin | gyrA | F: ATTCTGCCGAACGGATCGAG R: CGACCGCGTTATCCGAATTG |
51 | 459 | [20] |
| gyrB | F: TGGGCAACACCGAGGTGAAG R: ACGGGTCCATGGTGGTTTCC |
70 | 762 |
