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. 2023 Jan 9;12:e81792. doi: 10.7554/eLife.81792

Figure 2. Kindlin-2 loss causes dramatic hepatocyte apoptosis followed by enhanced proliferation of both biliary cells and hepatic stellate cells.

(a) TUNEL staining. Scale bars,100 μm. (b) Quantification of TUNEL-positive cells from 6 different fields from 6 mice per group. (c) Immunohistochemistry (IHC) staining for Caspase 3 was performed on paraffin liver sections of 4-week-old control and KO mice. Scale bars,100 μm. (d, e) Western blotting. Liver tissue lysates from 2-, 3-, and 4-week-old control and KO mice were subjected to western blotting analysis for expression of the indicated proteins. Gapdh was used as a loading control. (f, g) Ki67 staining. Liver sections from of 2-, 3-, and 4-week-old control and KO mice were subjected to Ki67 staining. Scale bars,100 μm. Quantification of (f). (h) IHC staining. Liver sections from 4-week-old control and KO mice were subjected to IHC staining for CK19. Scale bars,100 μm. Representative images showing a dramatic increase in the number CK19-positive cells in KO liver. (i, j) Immunofluorescence (IF) staining. Scale bars,100 μm. Liver sections from 4-week-old control and KO mice were subjected to IF staining for Desmin, a marker for hepatic stellate cells. Representative images showing high expression of Desmin in KO liver. Quantification of (i) from six mice per group. The results are shown as means ± SEM. *p<0.05, **p<0.01, vs control.

Figure 2—source data 1. Raw data related to Figure 2.

Figure 2.

Figure 2—figure supplement 1. Loss of Kindlin-2 resulted in increased apoptosis.

Figure 2—figure supplement 1.

(a) TUNEL staining shows increased number of apoptotic (yellow stain) cells in 4 weeks old KO liver. (b, c) Immunohistochemistry staining of different time liver sections from mice of the indicated genotypes for expression of active Caspase 3 (b) and p65 (c). Scale bars,100 μm.