Figure 7. Overexpression of Kindlin-2 ameliorates D-galactosamine/lipopolysaccharide (D-GalN/LPS)-induced liver injury and death.
(a, b) Eight-week-old C57BL/6 mice were intraperitoneally injected with D-GaIN/LPS (GalN, 700 mg/kg body weight, LPS, 3 mg/kg body weight) or PBS as controls for 5 hr. Liver tissues were collected and subjected to western blotting for expression of Kindlin-2. Quantitative data (a). N=6 mice/group. (c) Experimental design. (d, e) Western blotting. Eight-week-old C57BL/6 mice were first injected via tail vein with adeno-associated virus 8 (AAV8) (2×1011 particles/mouse) expressing Kindlin-2 (AAV8-K2) or GFP (AAV8-GFP). After 21 days, mice were then treated with D-GaIN/LPS for 5 hr. Protein extracts were then prepared from livers and subjected to western blotting for expression of Kindlin-2. Quantitative data (d). Gapdh was used as a loading control. N=3 mice per group. (f) Survival curve. Mice were treated as in (c), followed by observation for death. N=15 mice per group. (g) Gross liver appearance. Mice were treated as in (c). (h, i) ELISA assays. Serum ALT (h) and AST (i). Mice were treated as in (c). N=8 mice per group. (j) Hematoxylin and eosin staining in liver sections. Mice were treated as in (c). (k) Immunohistochemistry staining of Caspase-3 in liver sections. Mice were treated as in (c). (l, m) TUNEL staining of liver sections. Quantitation data (m). Mice were treated as in (c). N=6 mice per group. The results are shown as means ± SEM. **p<0.01, vs AAV-GFP.
Figure 7—figure supplement 1. Serum alanine transaminase (ALT) and aspartate transaminase (AST).
