Table 2.

Summary of the genes and primers isolated by SNP4Orphanspecies pipeline for the five tested species. * one per gene and not duplicated in the genome.

Species	Diplodiasapinea	Erysiphealphitoides	Armillariaostoyae	Thaumetopoeapityocampa	Bursaphelenchusxylophilus
Nb of Busco genes	3,786	3,234	3,870	5,286	3,131
Nb of Complete single-copy	3,733	2,353	3,787	2,219	2,068
Nb with the validated genus	3,557	987	3,765	NA	NA
Nb of defined amplicons	6,962	1,829	20,991	3,163	13,256
Nb of genes with amplicons	2,760	685	3,438	887	1,955
Nb of pairs of primers	6,095	1,408	20,617	1,916	10,938
Nb of conserved pairs of primers*	2,570	614	3,426	672	1,928
% gene duplication in pools	20.8	57.4	19	52.8	23