FIG. 7.

The spiral F-mixer has vastly superior mixing spatial distribution as compared to a diffusion-limited T-mixer. (a) A spiral pre-mixing sticker was retrofitted onto an existing microfluidic containing micropatterned proteins. (b) The T-mixer was laser cut in the same tape material as the spiral mixing sticker and adhered onto a pre-existing microfluidic device. (c) The spiral sticker successfully mixed the primary and secondary antibodies that subsequently enabled the visualization of all patterned proteins in the channel. (d) Conversely, the T-mixer only enabled the visualization of proteins along the middle of the channel as antibody mixing was diffusion limited. For both experiments, Re = 0.23.