FIG. 1.

Reactivity of β-Gal fusion proteins of AD clones with anti β-Gal antibody and sera from uninfected and M. tuberculosis-infected rabbits. Lysates (5 μg) of each AD lysogen and λgt11 vector lysogen were fractionated on SDS–10% PA gels, and Western blots were probed with anti-β-Gal antibody (lanes 2 to 5 in panel A and lanes 2 to 4 in panel B), a serum pool from uninfected rabbits (lanes 7 to 10 in panel A and lanes 6 to 8 in panel B), and a serum pool from infected rabbits (lanes 12 to 15 in panel A and lanes 10 to 12 in panel B). (A) Lanes 1, 6, and 11, molecular mass markers; lanes 2, 7, and 12, uninduced λgt11 lysogens; lanes 3, 8, and 13, induced λgt11 lysogens; lanes 4, 9, and 14, clone AD1; lanes 5, 10, and 15, clone AD2. (B) Lanes 1, 5, and 9, molecular mass markers; lanes 2, 6, and 10, clone AD9; lanes 3, 7, and 11, clone AD10; lanes 4, 8, and 12, clone AD16.