Skip to main content
. 2001 Jun;69(6):4185–4191. doi: 10.1128/IAI.69.6.4185-4191.2001

FIG. 3.

FIG. 3

Reactivity of β-Gal fusion proteins with human sera. Lysates (5 μg) of each AD lysogen and λgt11 vector lysogen were fractionated on SDS–10% PA gels, and Western blots were probed with human sera. (A) Clone AD9 (PE-PGRS). (B) Clone AD10 (PTRP). (C) Clone AD2 (PirG). (D) Clone AD16 (MtrA). Lanes 1, 4, 7, 10, and 13, molecular mass markers; lanes 2, 5, 8, 11, and 14, respective AD clones; lanes 3, 6, 9, 12, and 15, λgt11 vector lysogens. Lanes 2 and 3 were probed with anti-β-Gal antibody, lanes 5 and 6 were probed with pooled sera from PPD-positive healthy individuals, lanes 8 and 9 were probed with pooled preclinical TB sera from HIV-TB patients, lanes 11 and 12 were probed with pooled sera from noncavitary TB patients, and lanes 14 and 15 were probed with pooled sera from cavitary TB patients.