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. 2023 Jan 4;613(7944):582–587. doi: 10.1038/s41586-022-05560-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — Transformation efficiency of Target (T) and Non-Target (NT) plasmids into electrocompetent E. coli containing WT or mutant Cas12a2+3xcrRNA immune system plasmids shown by spot assay. Transformations were plated as 10x serial dilutions between 10¹ and 10⁶ and the number of colonies in the highest countable spot were used to determine the total number colony forming units (cfu) per 50 ng plasmid. Transformation fold reduction was calculated as -Log₁₀(T_cfu/NT_cfu). Active immune systems present a ≥100-fold difference between T and NT transformation efficiency. All transformations were completed to at least two biological replicates. For transformation plates source data, see Supplementary Fig. 1.