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. 2023 Jan 4;613(7944):575–581. doi: 10.1038/s41586-022-05528-w

Fig. 2. Dynamic concentration maps of Nups after anaphase onset.

Fig. 2

HeLa cells whose Nups are endogenously tagged with mEGFP or mCherry were imaged every 30 s by 3D confocal microscopy. Single confocal sections are shown. Images were calibrated by FCS to convert fluorescence intensities into cellular protein concentration (colour bars). Nup153 is heterozygously tagged and Nup358 and Pom121 are subphysiologically expressed (Extended Data Figs. 1 and 2). The bottom colour bar is for Nup153 and Pom121; the top colour bar is for the rest of the Nups. Images were filtered with a median filter (kernel size: 0.25 × 0.25 μm). Scale bar, 10 μm.