Fig. 4. Observation of single nuclear pores by super-resolution microscopy confirms the recruitment of Tpr precedes Nup62 in the interphase assembly pathway.
a,b, Three-dimensional STED imaging of Nup62–mEGFP genome-edited cells stained with a GFP nanobody and an anti-Tpr antibody at mid/late telophase (a) and interphase (b). Scale bars: left, 10 μm; right, 1 μm. The regions of the nuclear envelope indicated by white lines (left) are flattened and displayed (right). Nuclear pores that contain only Tpr or Nup62 are indicated by red and green arrows, respectively. c, The frequency of nuclear pores that contain both Tpr and Nup62, only Tpr or only Nup62 at each cell cycle stage. Data are from 10 cells for each stage. Data are mean ± s.d. ****P = 0.000017 (mid/late telophase) and ****P = 0.000080 (interphase), unpaired two-tailed t-test.