Extended Data Fig. 3. NPC assembly relies on and consumes almost half of the material inherited from the mother cell within one hour after mitosis.
FCS-calibrated 3D confocal microscopy was performed as in Fig. 2, and the Nup copy number was quantified in whole dividing cells (for details see Methods). The number of Nups in cytoplasm (dark blue), nucleoplasm (medium dark blue) and nuclear envelope (light blue) are plotted against time after anaphase onset. The plot is the mean of 15, 20, 13, 14, 22, 22, 19, and 24 cells for Nup107, Seh1, Nup205, Nup93, Nup62, Nup214, Tpr and Nup358, respectively.