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. 2022 Sep 8;41(1):96–107. doi: 10.1038/s41587-022-01410-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Overnight propagation assay to test the requirements of active intein splicing and stop codons to turn on or off, respectively, the SAC-PACE circuit. Inactive intein was generated by introducing the C1A mutation⁴¹ in the C-intein and the positive control (+ctrl) was a host strain containing pJC175e¹⁹. (b) Overnight propagation assay to test the linker length limitations of SAC-PACE, OT phage did not contain Nme2-ABE8e or TadA8e. (c) Overnight propagation assay to test the relative activity of Nme2-ABE8e phage when the target adenines within the stop codons are placed at different locations in the 23 nucleotide Nme2Cas9 protospacer (counting the PAM as positions 24-29). For (a-c), Mean±SEM is shown and are representative of n = 2 independent biological replicates. Fold-propagation is calculated as the ratio of titer after overnight propagation over inoculating titer.