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. 2022 Oct 10;41(1):140–149. doi: 10.1038/s41587-022-01475-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Tables showing statistical details of NF-κB transcriptional reporter activity in the absence and presence of selected viral proteins under unstimulated (top) and TNFα stimulated (bottom) conditions. One-way ANOVA with Dunnett’s multiple comparisons test, n = 3, adjusted P values are shown. b, Table showing statistical details of NF-κB transcriptional reporter activity at different amounts of transfected viral protein-encoded plasmid under unstimulated (left) and TNFα stimulated conditions (right). One-way ANOVA with Dunnett’s multiple comparisons test, n = 3 and n = 6, respectively, adjusted P values are shown. a and b, Raw data and full analysis is shown in Supplementary Table 9. c, Table showing statistical details of NF-κB transcriptional reporter activity under unstimulated (left), TNFα-stimulated (middle) and NSP14-induced conditions in WT and IKBKG KO HEK293 cells (two-way ANOVA with Dunnett’s multiple comparisons test, n = 3), adjusted P values are shown. d, Representative anti-IKBKG (top) western blot demonstrating levels of IKBKG in WT and three independent IKBKG knockout clones of HEK293 cells relative to actin beta (ACTB) loading controls (bottom). e, Representative anti-hemagglutinin (HA) western blot demonstrating levels of tagged NSP14 protein in NF-κB induction experiments relative to actin beta (ACTB) loading controls (bottom). f, Table showing statistical details of viral replication in wild-type, mock KO and CRISPR KOs of the indicated HuSCI host proteins. Kruskal-Wallis with Dunn’s multiple comparisons test, n = 9. Adjusted P values are shown. g, Cell viability of mock KO and CRISPR KOs of the indicated HuSCI host proteins relative to WT cells. Kruskal-Wallis with Dunn’s multiple comparisons test, n = 3. Adjusted, Fisher’s exact P values are shown. f and g, Raw data, Fisher’s exact P values, and full analysis is shown in Supplementary Table 10. h, Cell viability and relative replication of icSARS-CoV-2-nanoluciferase in HEK293 cells (left) and Vero E6 cells (right) at different concentrations of remdesivir. The EC50 values shown for each cell line were calculated with a variable slope model. Error bars: standard deviation of the mean, n = 3 biological repeats, full analysis in Supplementary Table 11.

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