Fig. 5.

Amorfrutin B post-treatment affected the expression levels of autophagy-related genes (a) and proteins (b), as measured using qPCR and ELISA. The results are presented as a fold change in case of qPCR and percentage of the control or pg/mg of protein ± SEM in case of ELISA. There were 3 independent experiments, consisting of 5–6 replicates per group. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001 compared to the control group, ^##p < 0.01, ^###p < 0.001 compared to the cultures exposed to hypoxia, ^{^}p < 0.05, ^{^^}p < 0.01, ^{^^^}p < 0.001 compared to the cultures exposed to ischemia