Table 1.

Multiple reaction monitoring conditions for analysis of 26 synthetic cathinones, dihydro-metabolites and  internal standard (MDPV-d₈) in whole blood by liquid chromatography–tandem mass spectrometry

Analyte number	Target analyte	Rt (min)	Precursor ion (m/z)	Product ion(s) (m/z)	CV (V)	CE (eV)	Dwell time (s)
1	Methylone	5.43	208	160	22	17	0.150
				132		25
2	Ethylone	5.82	222	174	24	19	0.050
				146		24
3	Methedrone	5.82	194	161	23	19	0.010
				176		12
4	Butylone	6.12	222	146	24	24	0.020
				204		13
5	Dihydro-mephedrone	6.08	180	131	24	18	0.050
				–		–
6	Dihydro-dibutylone	6.20	238	220	26	12	0.050
				–		–
7	Dibutylone	6.24	236	86	26	21	0.050
				191		15
8	Mephedrone	6.23	178	145	24	20	0.050
				160		12
9	4-CEC	6.78	212	159	27	18	0.050
				194		13
10	Dihydro-4-CEC	6.85	214	181	23	23	0.050
11	Dihydro-N-ethylpentylone	6.93	252	191	27	23	0.010
				–		–
12	Dihydro-4-EMC	6.96	194	117	26	22	0.010
13	4-Cl-α-PPP	7.01	238	139	30	27	0.010
				98		25
14	4-EMC	7.05	192	145	26	21	0.010
				174		12
15	N-ethylpentylone	7.05	250	202	27	18	0.010
				232		13
16	Dihydro-4-Cl-α-PPP	7.09	240	207	30	22	0.010
				–		–
17	α-PVP	7.14	232	91	35	25	0.010
				105		25
18	MDPV-d8	7.24	284	134	33	26	0.020
				–		–
19	MDPV	7.25	276	126	30	27	0.010
				135		24
20	Dihydro-4-MPD	7.29	208	147	25	22	0.010
				–		–
21	Dihydro-MDPV	7.32	278	217	30	22	0.010
				–		–
22	4-MPD	7.36	206	188	25	13	0.010
				145		20
23	N-Ethylhexedrone	7.57	220	202	27	14	0.010
				91		22
24	Dihydro-N-ethylhexedrone	7.65	222	147	27	23	0.010
				–		–
25	4-F-PHP	7.99	264	109	35	25	0.050
				140		30
26	4-Cl-α-PVP	8.01	266	125	31	21	0.050
				139		24
27	Dihydro-4-F-PHP	8.09	266	109	35	25	0.050
				–		–

Quantifier ions in bold

Rt retention time, CV cone voltage, CE collision energy