Fig. 2.

TNF-α treatment of trophoblasts and endothelial cells in 2D monocultures alters FKBPL and Gal-3 protein expression. a–c Western Blotting results of ACH-3Ps protein lysate expression of FKBPL and Gal-3. ACH-3Ps exposed to tumor necrosis factor alpha (TNF-α, 10 ng/mL) for 24 or 72 h. Control, untreated. GAPDH, loading control. d–f Western Blotting of HUVECs protein lysate showing expression of FKBPL and Gal-3. HUVECs exposed to TNF-α (10 ng/mL) for 24 or 72 h. Control, untreated GAPDH, loading control. Data passed Shapiro–Wilk normality test and were analyzed by one-way analysis of variance (ANOVA) with Tukey post-hoc test; n = 3; *p < 0.05, ***p < 0.001