Fig. 4.

The presence of ACH-3Ps cells and inflammatory conditions impacts vasculare network formation and FKBPL, Gal-3 and CD31 expression in endothelial cells cultured in a microfluidic device. a In endothelial cells monoculture microfluidic setting, HUVECs were combined with collagen matrix (2.5 mg/mL) and added to the central channel of the microfluidic chips. In the co-culture set of chips, HUVECs were embedded within the central matrix channel and ACH-3Ps were added to the side channel. Chips were treated with TNF-α (10 ng/mL) for 24 or 72 h, with untreated cells as a control. Following 72 h of culture, chips were probed for immunofluorescent imaging of FKBPL, CD31 and Gal-3. a Representative images of cells stained for DAPI, FKBPL and CD31. b Fold change of FKBPL, c Gal-3 and d CD31 expression in HUVECs without ACH-3Ps. e Fold change of FKBPL, f Gal-3 and g CD31expression in HUVECs with ACH-3Ps. Data presented as mean ± SEM, scalebar represents 100 µm. Unpaired student’s t test and ordinary one-way ANOVA with Tukey post hoc test for normally distributed data and Mann–Whitney or Kruskal–Wallis post hoc test for non-normally distributed data; n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001