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. 2023 Jan 18;13:975. doi: 10.1038/s41598-023-27770-6

Figure 2.

Figure 2

Functional evaluation of NONO variant in Drosophila. (a) Western blotting confirmed the expression of the NONO WT and the Pro459Ala variant. Myc-NONO WT or Myc-NONO Pro459Ala was expressed throughout Drosophila bodies and detected with anti-Myc antibody. Anti-α-tubulin was used as a loading control where indicated. (b) Representative bright-field microscope images of fly eyes displaying eye-specific ectopic expression of Myc-NONO WT and Myc-NONO Pro459Ala using the GMR-Gal4 driver, reared at 20 °C. (c) Quantification results of the phenotypic scores in control (n = 29), Myc-NONO WT (n = 32), and Myc-NONO Pro459Ala (n = 23). Data represent the mean ± SD. Statistical comparisons were conducted using nonparametric ANOVA (Kruskal–Wallis test) followed by Dunn’s multiple comparisons test. n.s.: not significant. ***P < 0.001, ****P < 0.0001. (d) The fly eyes displaying eye-specific overexpression of nonA using the GMR-Gal4 driver, reared at 29 °C. (e) Quantification results of the phenotypic scores in control (n = 21) and nonA (n = 19). Data represent the mean ± SD. Statistical comparisons were conducted using parametric test (unpaired t test with Welch’s correction). n.s.: not significant. ****P < 0.0001.