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. 2023 Jan 18;13:976. doi: 10.1038/s41598-023-27537-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differential Interference Contrast microscopy image of male larva recovered from NSG mice at 15 weeks post infection. Composite image of testis and reproductive tube. (a) C-shaped testis (arrow) at about midbody. (b) Highly coiled male reproductive tube (arrows) in posterior third of body. Scale bar 20 µm. Composite image of posterior end of male larva showing developing spicules. (c) Tail and one spicule (arrows) in lateral view showing size, shape and cuticularization. Cloaca and anal opening (arrow heads) also visible. (d) Dorso-ventral view showing both developing spicules (arrows). Scale bar 40 µm.