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. 2023 Jan 18;14:298. doi: 10.1038/s41467-023-35937-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a In vivo phenotypes after foxG (RNAi). Data representative of two independent experiments. b Immunostaining using α-SYNAPSIN (3C11) (neural system) and α-βCAT2 (β-catenin-2, digestive system) reveals rounded ventral nerve cords in foxG (RNAi) (tailless phenotype). Nuclei are stained in DAPI. WISH of posterior markers in regenerating foxG (RNAi) animals demonstrated a reduced expression. Data representative of two independent experiments. Schematic illustrations of posterior markers were added. c Immunostaining using α-SYNAPSIN (3C11) (neural system) reveals a posterior brain in the foxG (RNAi) two-headed animals. Data representative of two independent experiments. Nuclei are stained in DAPI. Posterior eyes are indicated with an orange arrow in a and c. Scale bar: 100 µm in a, immunostaining in b and c; 200 µm in WISH in b.