Table 2.
Protein expression of SLC and ABC transporters (fmol/μg crude membrane total protein) in crude membrane fraction of the brain cortices of female wild-type (WT) mice (n = 14) and 5xFAD mice (n = 12), as well as the comparison to the protein expression levels in crude membrane of the brain cortices in female WT rats (n = 5) versus TgF344-AD rats (n = 8) [28] and female transgenic APdE9 mice (n = 4) versus age-matched WT (n = 5) mice [29]
| Protein name/ gene name |
Mouse brain cortical tissue analysed in the present study |
Rat brain cortical tissue [28] |
Mouse brain cortical tissue | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| WT mice Mean ± SD |
5xFAD mice Mean ± SD |
Ratio 5xFAD to WT |
p-value | WT rats Mean ± SD |
TgF344-AD rats Mean ± SD |
Ratio TgF344-AD to WT |
p-value | WT mice | APdE9 mice | |
| ABC transporters | ||||||||||
| Abcb1/Abcb1a | 0.13 ± 0.061 | 0.15 ± 0.052 | 1.1 | 0.7 | 0.19 ± 0.059 | 0.17 ± 0.063 | 0.89 | 0.5 | 0.49 ± 0.19 | 0.31 ± 0.10 |
| Abcg2/Abcg2 | 0.46 ± 0.21 | 0.51 ± 0.24 | 1.1 | 0.7 | 0.038 ± 0.028 | 0.039 ± 0.019 | 1.1 | 0.9 | 0.40 ± 0.12 | 0.32 ± 0.070 |
| Abcc1/Abcc1 | 0.16 ± 0.033 | 0.16 ± 0.044 | 1.0 | 0.8 | 0.071 ± 0.024 | 0.062 ± 0.018 | 0.87 | 0.5 | 0.27 ± 0.070 | 0.24 ± 0.11 |
| Abcc4/Abcc4 | 0.019 ± 0.011 | 0.026 ± 0.011 | 1.4 | 0.1 | < 0.010 (ULQ) | < 0.010 (ULQ) | 0.020 ± 0.010 | 0.030 ± 0.010 | ||
| Abca1/ Abca1 | 0.12 ± 0.077 | 0.18 ± 0.13 | 1.5 | 0.3 | 0.031 ± 0.012 | 0.028 ± 0.0081 | 0.90 | 0.3 | NQ | NQ |
| SLC transporters | ||||||||||
| ASCT1/Slc1a4 | 3.1 ± 1.6 | 5.9 ± 2.5 | 1.9 | 0.01 | 0.12 ± 0.0029 | 0.075 ± 0.031 | 0.62 | 0.9 | NQ | NQ |
| GLUT1/Slc2a1 | 3.5 ± 1.6 | 3.8 ± 1.3 | 1.1 | 0.62 | 2.6 ± 0.14 | 1.8 ± 0.35 | 0.69 | 0.8 | 3.7 ± 1.1 | 3.1 ± 1.1 |
| 4F2hc/Slc3a2 | 0.81 ± 0.31 | 0.96 ± 0.38 | 1.2 | 0.4 | 0.53 ± 0.13 | 0.33 ± 0.13 | 0.62 | 0.5 | NQ | NQ |
| CAT-1/Slc7a1 | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | NQ | NQ | ||||
| LAT1/Slc7a5 | 0.73 ± 0.26 | 0.61 ± 0.18 | 0.84 | 0.3 | 0.065 ± 0.023 | 0.053 ± 0.021 | 0.82 | 0.3 | 0.25 ± 0.070 | 0.26 ± 0.10 |
| MCT1/Slc16a1 | < 0.10 (ULQ) | < 0.10 (ULQ) | < 0.10 (ULQ) | < 0.10 (ULQ) | NQ | NQ | ||||
| RFC/ Slc19a1 | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | NQ | NQ | ||||
| OATP1A4/ Slco1a4b | < 0.015 (ULQ) | < 0.015 (ULQ) | 0.16 ± 0.14 | 0.098 ± 0.069 | 0.61 | 0.5 | NQ | NQ | ||
| OATP1C1/ Slco1c1 | < 0.015 (ULQ) | < 0.015 (ULQ) | 0.072 ± 0.035 | 0.041 ± 0.037 | 0.57 | 0.1 | NQ | NQ | ||
| OCT1/ Slc22a1 | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | NQ | NQ | ||||
| OAT3/ Slc22a8 | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | < 0.15 (ULQ) | NQ | NQ | ||||
| FATP1/ Slc27a1 | 0.66 ± 0.39 | 0.83 ± 0.41 | 1.3 | 0.4 | 0.74 ± 0.21 | 0.76 ± 0.33 | 1.1 | 0.9 | NQ | NQ |
| ENT1/ Slc29a1 | < 0.03 (ULQ) | < 0.0 (ULQ) | < 0.010 (ULQ) | < 0.010 (ULQ) | NQ | NQ | ||||
| Plasma membrane marker | ||||||||||
| Na+/K+-ATPase | 72.0 ± 11.0 | 72.0 ± 16.0 | 1.0 | 0.9 | 46.0 ± 10.1 | 61.0 ± 14.0 | 1.3 | 0.06 | 170 ± 33.0 | 150 ± 42.0 |
Statistical significance of differences in transporter protein expression between groups was analyzed using unpaired t-test
NQ, not quantified referring to not investigated in the study; ULQ, under limit of quantification
aAbcb1 refers to both Abcb1a and Abcb1b
bOATP1A4 is an orthologue of human OATP1A2