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. 2023 Jan 19;14(1):39. doi: 10.1038/s41419-023-05580-y

Fig. 4. Activation of PKG I suppressed tumorigenesis and metastasis in vivo using an EOC xenograft model.

Fig. 4

A The xenograft models were generated after injected with A2780 cells and the administration of PBS or 8-Br-cGMP (5 or 20 mg/kg) in nude mice (n = 5/group). B The tumor weight was measured after the nude mice were euthanized. Error bars represent the means ± SD. C Weight changes of nude mice in different groups. D Images of hepatic metastasis (indicated by the red arrow) in nude mice injected with A2780 cells and the administration of PBS or 8-Br-cGMP (5 or 20 mg/kg). E The number of hepatic metastasis was counted after nude mice were euthanized. Error bars represent the means ± SD. F IHC analysis of EGFR, Ki-67, and MMP-9 in tumor tissues of nude mice. Scale bar: 100 μm (left), 50 μm (right). G Quantitative data on the expressions of EGFR, Ki-67, and MMP-9 in tumor tissues of nude mice. The results were presented as mean ± SD (n = 3). Significance was evaluated Ordinary two-way ANOVA analysis followed by Tukey’s multiple comparisons test in (B, E, G) and Two-way ANOVA followed by Tukey’s multiple comparisons test in (C). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 under indicated comparison.