Table 2.

Ionic strength dependence of DREB2A(244-272) variants binding to RCD1-RST(499-572).

	kon (µM−1 s−1)			koff (s−1)
NaCl	R266G	WT	D267L	R266G	WT	D267L
50 mM	413 ± 102	335 ± 64
100 mM	206 ± 49	245 ± 11	178 ± 35	0.70 ± 0.01	0.18 ± 0.01	0.030 ± 0.001
150 mM	175 ± 10	118 ± 4	149 ± 19
200 mM	120 ± 1	45 ± 2	142 ± 9		0.40 ± 0.01
300 mM	55.9 ± 0.3	36 ± 2	81 ± 3		0.53 ± 0.02
500 mM	21.0 ± 0.3	23.0 ± 0.2	45 ± 1		0.94 ± 0.01
800 mM	10.6 ± 0.3	8 ± 1	22 ± 1	5.29 ± 0.05	1.78 ± 0.02	0.223 ± 0.004
Basal rate	6 ± 2	5 ± 2	10 ± 2		5 ± 1

Recorded at 10 °C in 50 mM HEPES buffer, pH 7.4. Raw data available as Supplementary Data 1.

± Standard error from fit.