FIG. 3.

Genetic organization of the A. caviae Sch3N polar flagellin locus. Flagellar genes and ORFs are indicated by shaded boxes and named after their homologs in other bacterial species. Horizontal arrows indicate the direction of transcription. Open vertical arrows indicate the site of insertion of the KAN resistance cassette in the single mutants. The closed vertical arrow indicates the site of insertion of the KAN resistance cassette in the single mutant AA269 and the site of insertion of the CHL resistance cassette in the tandem mutant AAR31. Complementation plasmids based on the broad-host-range vector pBBR1MCS-3 are shown (see Table 1). The BalI (Ba), BglII (Bg), EcoRV (Ev), HincII (Hc), NaeI (N), PvuII (Pv), SacI (S), SmaI (Sm), and StuI (St) restriction sites are shown. Lollipop structures depict the approximate position of the putative transcriptional terminators.