Fig. 2.

Effects of tDCS-induced modulation of rotarod learning on the learning of beam walking. A Experimental protocol of beam walking. The mouse is subjected to anodal online tDCS as in Fig. 1C, except that the rotarod task is followed by a beam walking learning task in the absence of tDCS. Each mouse is familiarized to a wide beam (25 mm), followed by three trials on a thinner beam (days 1 and 2, 7 mm; days 3 and 4, 3 mm). B, C Data from dual-task experiments. B Average time on the rotarod is presented as in Fig.1C. C The average frequency of hindlimb slips is reduced during the 4-day training for beam walking. Note that online anodal tDCS during rotarod running improves rotarod learning (B), but has no effect on learning beam walking (C). n, total number of mice. D Summary of results showing learning rates for the rotarod and beam walking, as defined by normalized difference of the slip frequencies between the last and the first trials of walking on the 3-mm beam. E–G Learning the rotarod and beam walking with cathodal online (or offline) tDCS during rotarod learning. +, anodal tDCS; –, cathodal tDCS; 0, no current. Error bars, SEM; *P < 0.05, **P < 0.01, two-way ANOVA in B, C, E, F; unpaired t test in D, G.