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Locations of the real-time RT-RAA primers and probe on the M gene sequence of different IAVs strains. Use dots to represent nucleotide residues that match the majority. The forward primer (F91-121) is shaded in green; the reverse primer (R197-230) is blue and the exo probe (p122-170) is red. The two T residues within p122-170 labeled with a fluorophore (FAM) and quencher (BHQ1) are marked with solid and hollow triangles, respectively. THF is marked with arrow.
