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. 2023 Jan 5;12:1071288. doi: 10.3389/fcimb.2022.1071288

Figure 2.

Figure 2

Screening the optical primers for real-time RT-RAA detection. (A) Sketch map of primary primer screening. In the primer name, the numbers indicate the position within the M gene from H1N1 (GenBank accession no. KX879560.1). (B) Primary reverse primer screening results. The forward primer F2-31 was randomly selected to screen all five reverse primers. (C) Primary forward primer screening results. The picked reverse primer R202-231 was used to pick all five forward primers. (D) Sketch map of secondary primer screening. (E) Secondary reverse primer screening results. The picked forward primer F87-116 was applied to screen all seven reverse primers. (F) Secondary forward primer screening results. The picked reverse primer R197-230 was used to pick all seven forward primers.