Table 1.
The primers and probes used in IAVs real-time RT-RAA and RT-qPCR assays.
| Name | Sequences (5’→3’) | Gene | Product | Source |
|---|---|---|---|---|
| F91-121 | GTCTTTGCTGGGAAGAACACCGATCTTGAGG | M | 140 bp | This study |
| R197-230 | CTACGCTGCAGTCCTCGCTCACTGGGCACGGTGA | M | This study | |
| p122-170 a | CTCTCATGGAATGGCTAAAGACAAGACCAA(FAM-dT) (THF)C(BHQ1-dT)GTCACCTCTGACTAA[C3-spacer] | M | This study | |
| M-F | CTTCTAACCGAGGTCGAA ACG | M | 165 bp | Yang et al, 2022 |
| M-R | CTTTAGCCACTCCATGAGAGC | M | Yang et al, 2022 | |
| M-P b | FAM-CCTCAAAGCCGAGATC-MGB | M | Yang et al, 2022 |
a
FAM-dT, thymidine nucleotide carrying fluorescein; BHQ1-dT, thymidine nucleotide carrying black hole quencher 1; THF: tetrahydrofuran spacer; C3-Spacer, C3 spacer at the 3’ end to block elongation.
b
FAM, 6-carboxyfluorescein; MGB, minor groove binder.