FIG. 1.

Sequence analysis of the L. pneumophila pagP-like gene and PagP-like protein. (A) Sequence organization and restriction analysis of pEH12, pBOC16, and pTA2. For all plasmids, only the L. pneumophila strain 130b DNA fragment is depicted. Restriction sites are as follows: A, AflII; B, BamHI; E, EcoRI; N, NdeI; and S, SacI. (B) Alignment (boxed regions) of PagP-like proteins in L. pneumophila (Lpn), Salmonella serovar Typhimurium (Sty) (GenBank accession number AF057021), and E. coli (Eco) (GenBank accession number AE000167). The Clustal method was used to generate alignment data (45).