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In vivo competition assays with L. pneumophila 130b and NU260. The wild type (WT) and mutant were inoculated intratracheally into A/J mice (n = 4 to 6). The ratio of wild type to mutant in infected lungs was assessed by dividing the CFU per lung for the wild type by the CFU per lung for NU260. The values expressed are normalized to the deviation observed in the ratio of 130b to NU260 in the initial inoculum (i.e., 0.61).
