TABLE 2.
CAMP | Growth medium | Strain | MIC (μg/ml) | MBC (μg/ml) |
---|---|---|---|---|
PmB | BYE | 130b | 12.5 | 25–50 |
NU260 | 6.2 | 25 | ||
CDM | 130b | 3.1 | 25 | |
NU260 | 3.1 | 12.5–25 | ||
0.05 mM Mg2+–CDM | 130b | 6.2–12.5 | 25 | |
NU260 | 6.2 | 12.5–25 | ||
0.005 mM Mg2+–CDM | 130b | 12.5 | 25 | |
NU260 | 6.2 | 12.5–25 | ||
C18G | BYE | 130b | 16 | 32 |
NU260 | 8 | 16–32 | ||
CDM | 130b | 16 | 32 | |
NU260 | 4–8 | 8–16 | ||
0.05 mM Mg2+–CDM | 130b | 16 | 32 | |
NU260 | 4 | 8–16 | ||
0.005, mM Mg2+–CDM | 130b | 64 | 128 | |
NU260 | 16 | 16–32 |
The bacteria were grown in triplicate to mid-exponential phase in the indicated media and then, after dilution to 5 × 104 CFU/ml, were exposed to PmB or C18G. The incubation medium during all CAMP resistance assays was BYE. Similar MICs and MBCs were obtained in a replicate experiment (data not shown). A range of concentrations indicates the range of MICs of MBCs obtained from triplicate samples.