TABLE 3.

Advantages and disadvantages of different approaches to obtain MNs in culture.

	Small molecules	Transcription factors	Organ-on-chip	Organoids
Duration	Long term	Short term	Intermediate term	Long term
MN purity	Variable	High	Variable	Variable
Cost	Relative cheap	Relative cheap	Expensive	Expensive
Reproducibility	High variability	Low variability	Low variability	High variability
Maturation features	Absence of mature feature as single culture	Absence of mature feature as single culture	Improve of MN maturation in co-culture	Improve of MN maturation
Applicability	Drug screening	Drug screening	Drug screening including toxicity	Mechanism of cell-cell interaction
	Mechanism of a single cell	Mechanism of a single cell	Mechanism of cell-cell interaction
Scalability	Easy to scale up	Easy to scale up	Difficult to scale up	Difficult to scale up
Manipulation	Easy to handle	Easy to handle	Proper training needed	Proper training needed