Fig. 2.

Preparation and characterization of nanoparticles. (A) Polymerase chain reaction (PCR) map after transfection of 4T1 cells with NC-siRNA, siRNA-1, siRNA-2 and siRNA-3. (B) Western blot (WB) analysis of the changes in survivin expression induced by NC-siRNA, siRNA-1, siRNA-2 and siRNA-3. Particle size distribution (C), zeta potential (D), and TEM images (E) of nanogolds (Au), Au-DR-siRNA, and Au-DR-siRNA@LNPs. (F) The long-term stability of Au, Au-DR-siRNA, Au-DR-siRNA@LNPs at 4℃ for 8 weeks. The results were plotted as mean ± SD (n = 6). (G) Cy5 release curves from Au-DR-siRNA@LNPs in PBS with/without Dicer enzyme at pH 5.5, 6.5, and 7.5 during a 72 h incubation, respectively. The results were plotted as mean ± SD (n = 6). ***P < 0.001. (One-way ANOVA plus Tukey's multiple comparison).