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. 2022 Dec 7;18(1):100769. doi: 10.1016/j.ajps.2022.11.003

Fig. 3.

Fig 3

The screen of the feed ratio and the fluorescence recovery function of LNPs under Dicer enzyme. (A) Schematic for calculating the fluorescence quenching efficiency of Au-DR-siRNA. (B) Fluorescence spectrum of the supernatant obtained from Au and DR-siRNA at different feed ratios. (C) Fluorescence quenching efficiency of Au and DR-siRNA at different feed ratios. (D) Fluorescence spectrum of the supernatant after the incubation of Dicer with DR-siRNA, Au-DR-siRNA, Au-AQ-siRNA, and Au-DR-siRNA@LNPs for 5 min, respectively. (E) Fluorescence quantification of 4T1 cells treated with Au-AQ-siRNA@LNPs and Au-DR-siRNA@LNPs. The results were plotted as mean ± SD (n = 6). (F) CLSM images of 4T1 cells treated Au-AQ-siRNA@LNPs and Au-DR-siRNA@LNPs. Scale bar: 25 µm. (G) Schematic of lysosomal escape of Au-DR-siRNA@LNPs in tumour cells. (H) CLSM images of 4T1 cells indicating the lysosomal escape of Au-DR-siRNA@LNPs. Scale bar: 10 µm.