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. 2023 Jan 3;18:100534. doi: 10.1016/j.mtbio.2022.100534

Fig. 2.

Fig. 2

Evaluation of the antibacterial capacity of TPGS/Mup-Ag towards MuRSA and MuSSA in vitro. (A) MIC and (B) MBC susceptibility semiquantitative profiles of TPGS/Mup-Ag against MuRSA and MuSSA obtained by the microplate broth dilution method (n ​= ​3). (C) Growth curves of MuRSA and MuSSA incubated with different preparations (n ​= ​3). (D) Representative TEM images and (E) SEM images of MuRSA and MuSSA after incubating with different preparations for 6 ​h. (F) Fluorescence staining images of MuRSA and MuSSA after incubating with different preparations for 4 ​h. Live and dead bacteria were stained with calcein-AM (green fluorescence) and dead bacteria were stained with PI (red fluorescence). (G, H) Crystal violet staining and determination of the relative biofilm formation rate of MuRSA and MuSSA using a microplate reader upon OD590. Data are presented as the mean ​± ​SD: ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001.