Fig. 3.
In vitro antibacterial capacity in bacterial infection model. (A) Fluorescence images of HaCaT cells infected by MuRSA for 4 h, followed by treating with different preparations for 12 h. Live and dead cells were respectively stained with calcein-AM (green fluorescence) and dead cells were stained with PI (red fluorescence). (B) Representative TEM images of MuRSA-infected HaCaT cells (MOI = 20) after being treated with different preparations for 12 h. (C) The ratio of PI-positive cells to total cells in (A). (D) Survival rate of intracellular MuRSA cells after being treated with different preparations for 12 h (n = 3). (E) Rate of MuRSA adhesion to HaCaT after exposure to different preparations for 12 h (n = 3). Data are presented as the mean ± SD: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.