TABLE 1.
Cytokine and serum antibody responses to parasite antigen in six calves experimentally infected with O. ochengi, together with data from an uninfected animal
| Animal | IFN-γ concn (ng/ml)a
|
IL-4 (stimulation index)a
|
IL-2 concn (U/ml)a
|
IgG antibody concn (OD405)b
|
Infection intensityc
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Prepatentc | Patentd | Prepatent | Patent | Prepatent | Patent | Prepatent | Patent | Mf | Nod | |
| Infected | ||||||||||
| 704 | 614 | 104 | 5 | 3.1 | 2.8 | 1.7 | 0.46 | 0.57 | ND | ND |
| 706 | 121 | 16 | 4.9 | 3.4 | 2 | 2.1 | 0.64 | 0.96 | + | 1 |
| 707 | 568 | 21 | 5 | 1.5 | 2.4 | 0.7 | 0.49 | 0.82 | ++ | 7 |
| 708 | 228 | 9 | 6.3 | 2 | 4.8 | 1.2 | 0.41 | 0.78 | ++ | 4 |
| 709 | 608 | 54 | 4.9 | 3 | 1.8 | 2 | 0.48 | 0.78 | + | ND |
| 712 | 960 | 34 | 8.3 | 3.9 | 5.4 | 3 | 0.22 | 0.43 | + | 1 |
| Geometric mean | 421 | 29d | 5.6 | 2.7d | 2.9 | 1.6d | 0.42 | 0.70d | ||
| Control | ||||||||||
| 710 | 10 | 4 | 1.9 | 1.4 | 1.1 | 0.7 | 0.07 | 0.16 | ||
a
Cytokines were measured in culture supernatants of PBMC stimulated with antigen in vitro for 72 h. Shown are the mean values for all data points collected within the time interval. Prepatent, days 0 to 279 postinfection; patent, days >280 postinfection.
b
Mean value for all data points collected within time interval is shown. OD405, optical density at 405 n.m.
c
Mf, microfilarial density; ND, not detected; +, <1 microfilaria/mg of skin; ++, >1 microfilariae/mg of skin; Nod, number of O. ochengi nodules detected.
d
Significant difference between prepatent and patent values (P < 0.05).