TABLE 2.

Reactivity of monoclonal antibodies to VacA deletion mutants^a

Antibody	Reactivity to VacA antigen:
	Wild type	Δ6-27	Δ28-108	Δ56-83	Δ85-127	Δ112-196	Δ114-294	Δ517-536
9F2	+	+	+	+	+	+	+	+
5E4	+	+	+	+	+	+	+	+
5G5	+	+	+	+	+	+	−	+
1B6	+	+	+	+	+	+	+	−
B13O	+	+	+	+	+	+	+	−
4E	+	+	+	+	+	+	+	−
B3D	+	+	+	+	+	+	+	−
13B	+	+	−	−	−	−	−	+
B13B	+	+	−	−	−	−	−	+
C10F	+	+	−	−	−	−	−	+

^aH. pylori strains were grown in sulfite-free brucella broth containing 5% FBS, and the supernatant proteins were precipitated with a 50% saturated solution of ammonium sulfate. The VacA content of each sample was standardized so that each preparation yielded a similar optical density (OD) when reacted with polyclonal anti-VacA antiserum. +, OD₄₁₀ of > 0.2, which is >4-fold above the background; −, OD₄₁₀ of <0.2. Experiments were repeated three times with similar results.