TABLE 3.
Reactivity of monoclonal antibodies to VacA proteins from different wild-type H. pylori strainsa
| Antibody | Reactivity to VacA from H. pylori type and strain:
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| s1-m1
|
s1-m2
|
s2-m2
|
60190-vl (vacA-null mutant) | ||||||||||
| 60190 | 84-183 | 87-81 | 92-29 | 87-199 | 87-29 | 95-54 | 95-55 | 87-91 | Tx30a | 86-313 | 86-338 | ||
| 9F2 | + | + | + | + | + | + | − | − | − | − | − | − | − |
| 5E4 | + | + | + | + | + | + | − | − | − | − | − | − | − |
| 5G5 | + | + | + | + | + | + | + | − | + | − | − | − | − |
| 1B6 | + | + | + | + | − | + | − | − | − | − | − | − | − |
| B13O | + | + | + | + | − | + | − | − | − | − | − | − | − |
| 4E | + | + | + | + | − | + | − | − | − | − | − | − | − |
| B3D | + | + | + | + | − | + | − | − | − | − | − | − | − |
| 13B | + | − | + | + | + | + | − | + | − | − | − | − | − |
| B13B | + | − | + | + | + | + | − | + | − | − | − | − | − |
| C10F | + | − | + | + | + | + | − | + | − | − | − | − | − |
| Polyclonal anti-VacA | + | + | + | + | + | + | + | + | + | + | + | + | − |
a
H. pylori strains were grown in sulfite-free brucella broth containing 5% FBS to an optical density at 600 nm (OD600) of 0.4, and ELISA wells were coated with unconcentrated supernatants. The VacA content of each sample was confirmed by ELISA with pooled rabbit polyclonal anti-VacA antisera 958 (to 60190 VacA), 977 (to 95-54 VacA), and 957 (to 86-338 VacA). +, OD410 of >0.2, which is >4-fold above the background; −, OD410 of <0.2. Experiments were repeated three times with similar results.